DUTCH Plus

DUTCH Plus® Hormone Test

Comprehensive Hormone & Adrenal Assessment with Cortisol Awakening Response

The DUTCH Plus® is an advanced, next-generation hormone test that combines dried urine and saliva measurements for the most complete view of hormone health. It includes everything in the DUTCH Complete® plus a detailed look at the Cortisol Awakening Response (CAR) — the body’s natural stress response pattern that reveals how your brain and adrenals communicate upon waking.

This easy, at-home collection offers deep insight into hormonal balance, adrenal function, stress resilience, and sleep regulation — empowering you and your practitioner to tailor personalized treatment and lifestyle strategies.

What the DUTCH Plus Tests For

Sex Hormones & Metabolites

• Estrogens (E1, E2, E3) and estrogen metabolites (2-OH, 4-OH, 16-OH pathways)
• Progesterone metabolites (α-pregnanediol, β-pregnanediol)
• Testosterone and its metabolites (5α- and 5β-DHT)
• DHEA, androsterone, and etiocholanolone (androgen metabolism markers)

Adrenal Function & Cortisol Rhythm

• Free cortisol and cortisone (4-point saliva pattern throughout the day)
• Cortisol Awakening Response (CAR) via saliva (collected at waking, +30 min, +60 min)
• Metabolized cortisol and cortisone (total daily output)
• DHEA-S (key adrenal androgen marker)

Melatonin

• 6-Hydroxymelatonin sulfate (urinary marker for sleep and circadian rhythm regulation)

Organic Acids & Additional Biomarkers

• Oxidative stress marker (8-OHdG)
• Neurotransmitter metabolites (dopamine, norepinephrine, epinephrine pathways)
• Nutrient-related indicators (B-vitamin and methylation status)

Additional Insights Provided

• Estrogen, progesterone, and androgen balance ratios
• Phase I and II estrogen detoxification pathways
• Cortisol diurnal rhythm and awakening curve visualization
• Integration of urine and saliva data for complete HPA-axis analysis

 

Ideal For

• Fatigue, burnout, or stress intolerance
• Sleep disturbances or circadian rhythm issues
• PMS, PCOS, perimenopause, or menopausal symptoms
• Mood changes, anxiety, or low motivation
• Difficulty waking, morning fatigue, or “wired but tired” feeling
• Comprehensive hormone and adrenal assessment for functional medicine care